Happy first week back everyone! This week wasn't too eventful but involved
some vital planning for the next few weeks. Upon returning to UT, I was
surprised to hear that class was canceled because Dr.B's son was on the way
(congratulations!). But, nonetheless, the research had to go on. On
Wednesday,I added glycerol to my pNIC-Bsa4 + CA7 protein for long term
storage and double checked its concentration - 1.36 mg/ml (29.147 uM,
calculated using Beer's Law). Additionally, I verified the presence of
the master plate from which I originally got my protein and ensured correct
labeling and storage. On Wednesday, I also deliberated which direction to go
in - repeat my enzyme assay, grow up more protein, or Maxiprep the protein I
already had. Initially, I planned to grow up more protein but, realizing the
difficulty in stopping over the Labor Day weekend, I talked to super mentor
Adam and decided to Maxiprep instead. On Thursday,I began the Maxiprep and
started the overnight culture of my pNIC-Bsa with CA7. On Friday (tomorrow),
I will decant and spin down my overnight culture and store it in the -20C
fridge over the weekend, as well as organizing my notebook and making sure
everything is up to date. I plan to continue my Maxiprep next week, alongside
performing the refreshers Dr. B has made and beginning my virtual screening
(potentially).
Damir's Virtual Drug Screening Blog 